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Promega
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OriGene
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Image Search Results
Journal:
Article Title: A binding site for Pax proteins regulates expression of the gene for the neural cell adhesion molecule in the embryonic spinal cord
doi:
Figure Lengend Snippet: Mutations in PBS-1 and PBS-2 decrease N-CAM promoter activity in a N2A cell line stably expressing Pax-6. Cells were transfected with 0.5 μg of CMVβ and 2 μg of either a CAT gene construct driven by the −414/−15 N-CAM, a similar construct (designated −414/−15 M12) with mutations in PBS-1 and PBS-2, or the promoterless CAT gene (pCATbasic). CAT assays were performed in duplicate. The numbers refer to the relative levels of CAT activity produced by each construct.
Article Snippet: For promoter activation assays, a N2A neuroblastoma cell line permanently expressing Pax-6 was transfected with reporter gene constructs prepared in the
Techniques: Activity Assay, Stable Transfection, Expressing, Transfection, Construct, Produced